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human monocyte derived cd14 cells  (PromoCell)


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    PromoCell human monocyte derived cd14 cells
    Human Monocyte Derived Cd14 Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human monocyte derived cd14 cells/product/PromoCell
    Average 94 stars, based on 27 article reviews
    human monocyte derived cd14 cells - by Bioz Stars, 2026-02
    94/100 stars

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    (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of <t>iPSC-derived</t> macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.
    Human Induced Pluripotent Stem Cells Ipsc Derived Cd14 Monocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human monocyte derived cd14 cells  (PromoCell)
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    PromoCell human monocyte derived cd14 cells
    (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of <t>iPSC-derived</t> macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.
    Human Monocyte Derived Cd14 Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of <t>iPSC-derived</t> macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.
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    cell lines human ipsc cd14 monocytes atcc dys0100 human ipsc sensory neuron progenitors axol ax0555 experimental models  (ATCC)
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    (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of <t>iPSC-derived</t> macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.
    Cell Lines Human Ipsc Cd14 Monocytes Atcc Dys0100 Human Ipsc Sensory Neuron Progenitors Axol Ax0555 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    Image Search Results


    (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of iPSC-derived macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.

    Journal: Cell reports

    Article Title: Macrophage memories of early-life injury drive neonatal nociceptive priming

    doi: 10.1016/j.celrep.2024.114129

    Figure Lengend Snippet: (A) Example protein arrays of BMDM lysates with knockdown of p75NTR and stimulated with the indicated factors. (B) Heatmap indicating protein expression detected across each stimulus; n = 4/group. (C) Examples of significantly regulated proteins from the arrays (* p < 0.05 vs. siCON+Veh, ^ p < 0.05 vs. sip75+LPS, # p = 0.05 vs. sip75+Veh, ## p > 0.05 vs. sip75+Veh; one-way ANOVA, Tukey’s or ANOVA on ranks, Dunn’s; n = 4/group). (D–F) Representative images of iPSC-derived macrophages, sensory neurons, and Rhod-2 responses to ATP. Arrows, increased responses; arrowheads, new responses. Scale bar, 25 μm. (G) Example calcium transients from iPSC-derived sensory neurons in response to ATP, capsaicin, or KCl. Arrows indicate when the ATP or capsaicin was added, and the brackets indicate the part of the trace that was analyzed to calculate responses. scale bar, 20 μM. (H) Exposure of iPSC-derived sensory neurons to medium from macrophages with loss of p75NTR treated with NGF caused decreased responsiveness of neurons to ATP (F = 4.257) (* p < 0.04 vs. all other conditions, one-way ANOVA with Tukey’s post hoc, n = 15 cells per group). (I) No changes in capsaicin responses (F = 2.702) were observed (one way ANOVA). Mean ± SEM or percent change from controls with variance.

    Article Snippet: Human induced pluripotent stem cells (iPSC) derived CD14 + monocytes (ATCC, Manassas, VA, Cat No. DYS0100) were plated in macrophage differentiation medium (RPMI-1640 Medium/10% Fetal Bovine Serum (FBS), supplemented with 100 ng/mL human M-CSF, and 1% Pen/Strep) in 24 well plate at a density of 10 ^ 5 cells/ml according to manufacturer’s directions.

    Techniques: Knockdown, Expressing, Derivative Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Macrophage memories of early-life injury drive neonatal nociceptive priming

    doi: 10.1016/j.celrep.2024.114129

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human induced pluripotent stem cells (iPSC) derived CD14 + monocytes (ATCC, Manassas, VA, Cat No. DYS0100) were plated in macrophage differentiation medium (RPMI-1640 Medium/10% Fetal Bovine Serum (FBS), supplemented with 100 ng/mL human M-CSF, and 1% Pen/Strep) in 24 well plate at a density of 10 ^ 5 cells/ml according to manufacturer’s directions.

    Techniques: Recombinant